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JoVE Journal
Biology

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Flash Freezing and Cryosectioning E12.5 Mouse Brain
 
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Flash Freezing and Cryosectioning E12.5 Mouse Brain

Article DOI: 10.3791/198-v 28:32 min May 28th, 2007
May 28th, 2007

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Summary

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Click here for the English version.

Demonstrated in this video are the techniques for flash freezing and sectioning embryonic brain tissue from mouse. Useful tips for using the cryostat are given, including troubleshooting methods that can be used while cutting to ensure that the resultant tissues sections are free of cracks and other distortions.

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Flash Freezing Cryosectioning E12.5 Mouse Brain
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