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Establishing Intracranial Brain Tumor Xenografts With Subsequent Analysis of Tumor Growth and Response to Therapy using Bioluminescence Imaging
This content is Free Access.
Chapters
- 00:00Title
- 00:57Introduction
- 01:19Tumor Cell Preparation
- 02:44Tumor Cell Implantation
- 06:51Bioluminsecence Monitoring of Tumor Growth
- 07:49Image Analysis
- 08:59Brain Excision and Fixation
- 09:22Representative Results
- 10:39Conclusion
Luciferase-modified human brain tumor xenografts can be established intracranially in athymic mice, with subsequent monitoring of tumor growth and response to therapy using bioluminescence imaging. In combination with survival analysis, bioluminescence monitoring is an essential research tool for pre-clinical testing of therapies being considered for treating brain tumors.
Tags
Intracranial Brain Tumor XenograftsTumor GrowthTherapy ResponseBioluminescence ImagingTransplantation ModelsNeuro-oncology ResearchHuman Tumor XenograftSubcutaneous InjectionImmunocompromised MiceOrthotopic Xenograft EstablishmentNeuroanatomical StructuresIntracranial Xenograft EstablishmentTumor BurdenNon-invasive ImagingBioluminescence MonitoringOrthotopic Brain Tumor EstablishmentExperimental Therapies
