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JoVE Journal Cancer Research

Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts (Video) | JoVE

Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples

Journal (Cancer Research)

Harnessing the DNA Dye-triggered Side Population Phenotype to Detect and Purify Cancer Stem Cells from Biological Samples

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Journal (Cancer Research)

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts (Video) | JoVE

Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts from Fresh Tissue

Article DOI: 10.3791/200204-v • 05:38 min • May 26th, 2023

May 26th, 2023 •

Jesús Frutos Díaz-Alejo*^1,2,3,4, Simon April-Monn*⁵, Marina Cihova⁶, Verona Buocikova⁶, Jorge Villalón López^1,3, Maria Urbanova⁶, Carmen G. Lechuga⁷, Miroslav Tomas^6,8, Peter Dubovan^6,8, Bárbara Luna Sánchez³, Sonia Camaño Páez³, Alfonso Sanjuanbenito^2,9, Eduardo Lobo⁹, Estefanía Romio de la Heras¹⁰, Carmen Guerra^2,7, Carolina de la Pinta¹¹, Emma Barreto Melian^1,2, Mercedes Rodríguez Garrote^1,2, Alfredo Carrato^1,2,4, Laura Ruiz-Cañas^3,12,13, Bruno Sainz, Jr.^2,3,12,13, Ana Torres³, Bozena Smolkova⁶, Julie Earl^1,2,3

¹Molecular Epidemiology and Predictive Tumor Markers Group, Area 3, Ramón y Cajal Health Research Institute (IRYCIS), ²The Biomedical Research Network in Cancer (CIBERONC), ³Biobank and Biomodels Platform (PT20/0045), ISCIII research and development platforms in biomedicine and health sciences, BioBank Hospital Ramón y Cajal-IRYCIS, Spanish National Biobanks Network (ISCIII Biobank Register No. B.0000678), Ramón y Cajal Health Research Institute (IRYCIS), ⁴Faculty of Medicine, University of Alcalá de Henares, ⁵Institute of Tissue Medicine and Pathology, University of Bern, ⁶Department of Molecular Oncology, Cancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, ⁷Experimental Oncology, Molecular Oncology Program, Centro Nacional de Investigaciones Oncológicas (CNIO), ⁸Department of Surgical Oncology, National Cancer Institute, Slovak Medical University, ⁹Pancreatic and Biliopancreatic Surgery Unit, Hospital Universitario Ramón y Cajal, ¹⁰Department of Pathology, Hospital Universitario Ramón y Cajal, ¹¹Department of Radiation Oncology, Hospital Universitario Ramón y Cajal, ¹²Department of Cancer, Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), ¹³Cancer Stem Cell and Fibroinflammatory Group, Chronic Diseases and Cancer, Area 3, IRYCIS

* These authors contributed equally

Tags

Cancer Research Fibroblasts Ex Vivo Tumor Models Primary Tumor Tissues Translational Cancer Research Treatment Sensitivity And Resistance Cell-cell Interactions Tumor Microenvironment Culture Techniques Growth Factor Cocktails Biological Basement Membrane Tissue Sample Collection Biobanking And Storage Laboratory Capabilities SOP/protocol Pancreatic Adenocarcinoma Patient-derived Xenografts (PDX) Tissue Culture Mouse Facilities

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