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JoVE Encyclopedia of Experiments
Encyclopedia of Experiments: Biology

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Open Field Behavior Test: A Method to Assess General Locomotion and Exploration Habits



Perform the open field behavior test in a square box large enough to accommodate free movement of the test animal and with walls high enough to prevent the animal's escape. Mount a camera above the box so that the entire open field space is in the field of view and can be recorded.

Place a test animal, such as a mouse, in the center of the arena and begin the recording. To assess the animal's locomotion and exploration habits, track its behavior while it moves freely and explores the environment. Once the trial is completed, remove the mouse and clean the lining of the box.

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