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- On a positively-charged microscope slide, adhere two cover slips with enough distance between them to create a space for the Drosophila brains to be mounted. Under the stereo microscope, pipette the brains in mounting medium into that space. The negative charge of the tissue will help the brains adhere to the positive charge on the surface of the slide.
Remove excess mounting medium to allow for precise positioning of the brains on the slide in a grid pattern with the antennal lobes facing up, then bridge the two cover slips by adhering a third cover slip across to cover the brains.
To fill in the cavity, place mounting medium one drop at a time into the edge of the cavity, and allow it to spread by capillary motion so that it does not disturb the brains.
Seal the cavity with nail polish, and store at negative 20 degrees Celsius in the dark to preserve the fluorescence of stained tissues.
In the example protocol, we will mount Drosophila brains for confocal imaging of immunostained neurons.
- To mount the brains, build a bridge slide. Position two base covers slips roughly one centimeter apart on a positively-charged slide. Make certain that the positively-charged side is face up
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