Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Preparation of Mouse Brain Tissue for…
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

JoVE Journal Neuroscience
Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples
Journal (Neuroscience)
Measuring Post-Stroke Cerebral Edema, Infarct Zone and Blood-Brain Barrier Breakdown in a Single Set of Rodent Brain Samples
Injection of Glioblastoma (GBM) Cells into E5 Optic Tectum In Ovo (Video) | JoVE
Journal (Neuroscience)
Injection of Glioblastoma (GBM) Cells into E5 Optic Tectum In Ovo (Video) | JoVE
 
Click here for the English version

Preparation of Mouse Brain Tissue for Immunoelectron Microscopy

Article DOI: 10.3791/2021-v • 08:47 min • July 20th, 2010
July 20th, 2010 •
1, 2, 1
1Department of Neurobiology and Anatomy, University of Rochester, 2Douglas Mental Health University Institute

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.

Tags

Mouse Brain Tissue Immunoelectron Microscopy Transmission Electron Microscopy TEM Microglial Oligodendrocytic Astrocytic Neuronal Subcellular Compartments Dendrite Dendritic Spine Axon Axon Terminal Perikaryon Intracellular Organelles Cytoskeleton Pre-embedding Immunocytochemistry Antibody Iba1 Marker Neurotransmitter Contents Ultrastructural Localization Of Proteins Acrolein Fixative Transcardiac Perfusion Brain Sectioning Immunoperoxidase-diaminobenzidine Staining Resin Embedding Ultrathin Sectioning
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter