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Journal / Biology / DNA Stabil-Isotope Probing (DNA-SIP)
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DNA Stabil-Isotope Probing (DNA-SIP)

Article DOI: 10.3791/2027-v • 14:58 min • August 2nd, 2010
August 2nd, 2010 •
1, 1
1Department of Biology, University of Waterloo

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DNA stabil-isotop sondering är en odlings-oberoende metod för att identifiera och karakterisera aktiva samhällen av mikroorganismer som kan utnyttja vissa substrat. Assimilering av substrat anrikat med tunga isotop leder till inkorporering av märkta atomer till mikrobiell biomassa. Densitetsgradient ultracentrifugering hämtar märkt DNA för nedströms molekylära analyser.

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Mikrobiologi DNA stabila isotopen-sondering mikrobiologi mikrobiell ekologi odling oberoende metagenomik 16S rRNA genen gemenskap analys substrat mikrobiell ekologi anrikning
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