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Biology
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DNA的稳定同位素探测(DNA的SIP)
JoVE Journal
Biology
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JoVE Journal
Biology
DNA Stable-Isotope Probing (DNA-SIP)
Please note that all translations are automatically generated.
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DNA的稳定同位素探测(DNA的SIP)
DOI:
10.3791/2027-v
•
14:57 min
•
August 02, 2010
•
Eric A. Dunford
,
Josh D. Neufeld
1
Department of Biology
,
University of Waterloo
Chapters
00:04
Title
01:07
Introduction
02:11
Sample Incubation and DNA Extraction
03:47
Gradient Solns for Ultracentrifugation
05:26
Ultracentrifugation
07:49
Gradient Fractionation
09:46
DNA Precipitation
11:22
Representative Results
14:04
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
DNA的稳定同位素探测,识别和特性的微生物能够利用特定的基板活跃的社区是一个独立的种植方法。基板同化富集重同位素的信息纳入到微生物生物量的标记原子。密度梯度超速离心检索下游分子生物学分析的DNA标记。
Tags
DNA-SIP
Stable-isotope Probing
Microorganisms
Carbon Substrates
Nutrients
Cellular Biomass
Cultivation-independent Technique
Metabolic Function
Terrestrial Environments
Aquatic Environments
Stable-isotope Labelled Compounds
Nucleic Acid
Density Gradient Ultracentrifugation
Gradient Fractionation
DNA Purification
Cesium Chloride
Molecular Characterization
Fingerprinting
Microarrays
Clone Libraries
Metagenomics
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