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Primer-Free Aptamer Selection mit einem zufälligen DNA-Bibliothek
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Primer-Free Aptamer Selection mit einem zufälligen DNA-Bibliothek

Article DOI: 10.3791/2039-v • 11:14 min • July 26th, 2010
July 26th, 2010 •
1, 1, 1, 2, 2, 3,4
1Department of Pathology, Hershey Medical Center, Pennsylvania State University, 2Department of Chemistry, Pennsylvania State University, 3Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center, Pennsylvania State University, 4Materials Research Institute, Pennsylvania State University

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SELEX-Protokolle umfassen mehrere Runden der Selektion, von denen jeder Regeneration von Liganden, die wiederum erfordern feste Primersequenzen flankierenden die zufällige Bibliothek Regionen erfordern. Diese festen Primersequenzen können mit der Auswahl (false positive und negative) stören. Hier präsentieren wir eine Grundierung-free-Protokoll.

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Cellular Biology Ausgabe 41 Aptamer Auswahl S100B Sandwich
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