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Primer-Free aptamer Val användning av ett slumpmässigt DNA-bibliotek
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Primer-Free aptamer Val användning av ett slumpmässigt DNA-bibliotek

Article DOI: 10.3791/2039-v • 11:14 min • July 26th, 2010
July 26th, 2010 •
1, 1, 1, 2, 2, 3,4
1Department of Pathology, Hershey Medical Center, Pennsylvania State University, 2Department of Chemistry, Pennsylvania State University, 3Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center, Pennsylvania State University, 4Materials Research Institute, Pennsylvania State University

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SELEX protokoll omfattar flera omgångar av urval, var och en som kräver förnyelse av bundna ligander, vilket i sin tur kräver fasta primer sekvenser flankerar slumpmässiga biblioteket regionerna. Dessa fasta primer sekvenser kan störa urvalsprocessen (falskt positiva och negativa). Här presenterar vi en primer-fri protokollet.

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Cellbiologi aptamer urval S100B smörgås
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