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Immunology and Infection

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Non-invasive Imaging of Leukocyte Homing and Migration in vivo

Article DOI: 10.3791/2062 • 07:43 min • December 5th, 2010
December 5th, 2010 •
1, 1,2, 1, 1, 1
1Department of Pathology and Immunology, Washington University in St. Louis, 2National Institute of Neurological Disorders and Stroke, NINDS, NIH - National Institute of Health

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Summary
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Here, we describe a non-invasive two-photon (2P) microscopy approach to study leukocyte homing in the mouse footpad. We discuss the technical aspects of our tissue imaging preparation and walk the reader through a typical experiment from initial set up to execution and data collection.

Tags

Non-invasive Imaging Leukocyte Homing Leukocyte Migration In Vivo Imaging Two-photon Microscopy High Resolution Imaging Cell Behavior Intact Tissues Live Mice Leukocyte Recruitment Host Defense Infection Inflammatory Diseases Autoimmune Diseases Intravital Imaging Surgical Preparation Blood Vessels Tissues Tissue Damage Inflammation Non-invasive Single-cell Imaging Leukocyte Trafficking Mouse Footpad And Phalanges
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