Non-invasive Imaging of Leukocyte Homing and Migration in vivo

Leukocyte recruitment plays a significant role in host defense against infection, and one unchecked can contribute to inflammatory and autoimmune diseases. Here leukocyte recruitment and migration is visualized by first injecting quantum dots into a mouse to fluorescently labeled the blood vessel lumen, then injecting bacteria into the footed subcutaneously. The mouse is then transferred to a prewarm stage where its paw is secured to the cover glass of the imaging chamber.

Images of in vivo neutrophil recruitment and migration are then acquired using two photon microscopy and the various steps in leukocyte recruitment from rolling and adhesion to diapedesis and chemotaxis are quantitatively analyzed. Hi, I'm Wan from Dr.Mark Miller's in the Department of Pathology and Immunology at Washington University in St.Louis. Today we'll show you a procedure for non-invasive two photon imaging of leukocyte, homing and migration in vavo.