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ΓH2AX quantificazione dei Foci, a campioni di tessuto
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ΓH2AX quantificazione dei Foci, a campioni di tessuto

Article DOI: 10.3791/2063-v • 08:48 min • June 28th, 2010
June 28th, 2010 •
1,2, 1,3, 1,2,3, 1, 2,3, 3,4,5, 1,3
1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 2Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 3Department of Pathology, The University of Melbourne, 4Department of Allergy and Immunology, Murdoch Children's Research Institute, Royal Children's Hospital, 5Department of Pediatrics, The University of Melbourne

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La quantificazione di DNA a doppio filamento sulla base di γH2AX focolai è diventato uno strumento prezioso, in particolare in biologia radiazione, per la valutazione della radiosensibilità dei tessuti e gli effetti dei composti radiazione modificando. Qui mostriamo l'utilizzo di un test di immunofluorescenza per la quantificazione del γH2AX focolai in campioni di tessuto.

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Biologia Cellulare Numero 40 immunofluorescenza DNA a doppio filamento variante istone H2AX danni al DNA le radiazioni ionizzanti specie reattive dell'ossigeno
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