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Microiniezioni endovenosa di larve Zebrafish allo Studio danno renale acuto
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Microiniezioni endovenosa di larve Zebrafish allo Studio danno renale acuto

Article DOI: 10.3791/2079-v • 15:56 min • August 4th, 2010
August 4th, 2010 •
1, 2, 3, 1
1Department of Developmental Biology, University of Pittsburgh, 2Department of Biological Sciences, University of Pittsburgh, 3Department of Medicine and Genetics, Harvard Medical School

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Descriviamo una tecnica di microinjecting l'aminoglicoside, gentamicina, in 2 giorni dopo fetilization (DPF) larve di zebrafish per indurre danno renale acuto (AKI). Abbiamo anche descritto un metodo per intero immunoistochimica montaggio, plastica embedding e sezionamento delle larve di zebrafish per visualizzare il danno mediato AKI.

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Biologia dello Sviluppo Numero 42 microiniezione per via endovenosa zebrafish gentamicina danno renale acuto
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