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Journal / Biology / 급성 신장 상해를 공부하기 위해 Zebrafish 애벌레의 정맥…
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Biology

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JoVE Journal Biology
급성 신장 상해를 공부하기 위해 Zebrafish 애벌레의 정맥 Microinjections
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급성 신장 상해를 공부하기 위해 Zebrafish 애벌레의 정맥 Microinjections

Article DOI: 10.3791/2079-v • 15:56 min • August 4th, 2010
August 4th, 2010 •
1, 2, 3, 1
1Department of Developmental Biology, University of Pittsburgh, 2Department of Biological Sciences, University of Pittsburgh, 3Department of Medicine and Genetics, Harvard Medical School

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우리는 이일 이후 fetilization (DPF) 급성 신장 손상 (아키)를 유도하기 위해 zebrafish의 유충에 aminoglycoside, gentamicin을 microinjecting의 기술을 설명합니다. 우리는 또한 전체 탑재 immunohistochemistry하는 방법을 설명, 플라스틱 포함하고 아키 인한 손상을 시각화하기 위해 zebrafish 애벌레의 sectioning.

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발달 생물학 제 42 정맥 microinjection zebrafish gentamicin 급성 신장 손상
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