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Test för att mäta aktiviteten hos Escherichia coli Inducible Lysin Decarboxyase
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Test för att mäta aktiviteten hos Escherichia coli Inducible Lysin Decarboxyase

Article DOI: 10.3791/2094-v • 08:09 min • December 19th, 2010
December 19th, 2010 •
1, 1
1Department of Biochemistry, University of Toronto

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Aktiviteten i den inducerbara lysin decarboxylase övervakas genom att reagera substratet L-lysin och produkten cadaverine med 2,4,6-trinitrobenzensulfonic syra och bildar addukter som har differentiell löslighet i toluen.

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Biokemi inducible Lysin Decarboxyase Acid Stress stränga Response Pyridoxal-5'-fosfat beroende decarboxylase guanosin 5'-difosfat 3'-difosfat
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