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Blaue Native Polyacrylamid-Gelelektrophorese (BN-PAGE) zur Analyse von Multiproteinkomplexen aus Zelllysaten
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Blaue Native Polyacrylamid-Gelelektrophorese (BN-PAGE) zur Analyse von Multiproteinkomplexen aus Zelllysaten

Article DOI: 10.3791/2164-v • 12:04 min • February 24th, 2011
February 24th, 2011 •
1,2,3, *2,3, *3
1Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, 2Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology, University of Freiburg, 3Department of Molecular Immunology, Max-Planck-Institute of Immunology and Epigenetics
* These authors contributed equally

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In diesem Video, beschreiben wir die Charakterisierung von Multiproteinkomplexen (MPL) durch blaue nativen Polyacrylamidgelelektrophorese (BN-PAGE). In einer ersten Dimension sind dialysiert Zelllysaten durch BN-PAGE getrennt an die einzelnen MPL zu identifizieren. In einer zweiten Dimension SDS-PAGE, werden MPC von Interesse weiter unterteilt, um ihre Wähler durch Immunoblot analysieren.

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Biochemie BN-PAGE 2D-Gelelektrophorese Zelllysat Dialyse Protein-Komplex Multiproteinkomplex Protein-Wechselwirkung
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