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Blu nativi poliacrilammide gel elettroforesi (BN-PAGE) per l'analisi di complessi multiproteici da lisati cellulari
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Blu nativi poliacrilammide gel elettroforesi (BN-PAGE) per l'analisi di complessi multiproteici da lisati cellulari

Article DOI: 10.3791/2164-v • 12:04 min • February 24th, 2011
February 24th, 2011 •
1,2,3, *2,3, *3
1Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, 2Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology, University of Freiburg, 3Department of Molecular Immunology, Max-Planck-Institute of Immunology and Epigenetics
* These authors contributed equally

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In questo video, si descrive la caratterizzazione di complessi multiproteici (PPM) di blu elettroforesi su gel di poliacrilammide nativo (BN-PAGE). In una prima dimensione, dializzati lisati cellulari sono separati da BN-PAGE per identificare i singoli PPM. In una seconda dimensione SDS-PAGE, PPM di interesse sono ulteriormente suddivisi per analizzare i loro elettori mediante immunoblotting.

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Biochimica Numero 48 BN-PAGE elettroforesi su gel 2D lisato cellulare dialisi complesso proteico complesso multiproteico l'interazione delle proteine
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