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Blauwe Inheemse polyacrylamidegelelektroforese (BN-PAGE) voor de Analyse van multiprotein complexen van Cellular Lysaten
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Blauwe Inheemse polyacrylamidegelelektroforese (BN-PAGE) voor de Analyse van multiprotein complexen van Cellular Lysaten

Article DOI: 10.3791/2164-v • 12:04 min • February 24th, 2011
February 24th, 2011 •
1,2,3, *2,3, *3
1Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, 2Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology, University of Freiburg, 3Department of Molecular Immunology, Max-Planck-Institute of Immunology and Epigenetics
* These authors contributed equally

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In deze video, beschrijven we de karakterisering van multiprotein complexen (MPL), door blauwe inheemse polyacrylamide gel elektroforese (BN-PAGE). In een eerste dimensie, worden gedialyseerd cellulaire lysaten gescheiden door BN-PAGE om individuele Mediterrane partnerlanden te identificeren. In een tweede dimensie SDS-PAGE, zijn mediterrane partnerlanden van belang verder onderverdeeld naar hun kiezers te analyseren door immunoblotting.

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Biochemie BN-PAGE 2D-gelelektroforese cellulaire lysaat dialyse eiwitcomplex multiprotein complex eiwit interactie
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