Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Blå Native polyakrylamidgelelektrofores (BN-PAGE) …
JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology
Blå Native polyakrylamidgelelektrofores (BN-PAGE) för analys av Multiproteinkomplex från Cellular Lysates
Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
Journal (Biology)
Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA In Situ Hybridization Assay
Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping
Journal (Biology)
Ice-Cap: A Method for Growing Arabidopsis and Tomato Plants in 96-well Plates for High-Throughput Genotyping
 
Click here for the English version

Blå Native polyakrylamidgelelektrofores (BN-PAGE) för analys av Multiproteinkomplex från Cellular Lysates

Article DOI: 10.3791/2164-v • 12:04 min • February 24th, 2011
February 24th, 2011 •
1,2,3, *2,3, *3
1Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, 2Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology, University of Freiburg, 3Department of Molecular Immunology, Max-Planck-Institute of Immunology and Epigenetics
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

I denna video beskriver vi karakterisering av Multiproteinkomplex (Medelhavsländerna) med blå infödda polyakrylamidgelelektrofores (BN-sida). I en första dimension är dialyseras cellulära lysates åtskilda av BN-PAGE att identifiera enskilda partnerländerna. I en andra dimension SDS-PAGE är Medelhavsländerna av intresse indelade för att analysera sina väljare genom att immunoblotting.

Tags

Biokemi 48 BN-PAGE 2D gelelektrofores cellulära lysat dialys proteinkomplex Multiproteinkomplex komplexa proteininteraktioner
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter