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JoVE Journal
Biology

This content is Open Access.

Isolation of Stem Cells from Human Pancreatic Cancer Xenografts
 

Isolation of Stem Cells from Human Pancreatic Cancer Xenografts

Article DOI: 10.3791/2169
September 26th, 2010

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Summary September 26th, 2010

Cancer stem cells (CSCs) have been identified in a number of malignancies. In this protocol we describe a flow cytometric method utilizing aldehyde dehydrogenase activity and CD44 and CD24 expression to isolate CSCs from human pancreatic adenocarcinoma xenografts. These viable cells can then be used in functional and analytical studies.

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