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Gene consegna di cervello di ratto post-natale da parte di non-ventricolare iniezione plasmidi e Elettroporazione
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Gene consegna di cervello di ratto post-natale da parte di non-ventricolare iniezione plasmidi e Elettroporazione

Article DOI: 10.3791/2244
September 17th, 2010 •
1, 1,2, 1,2, 1
1Neuroscience Center, University of Helsinki, 2Faculty of Biological and Enviromental Sciences, University of Helsinki

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September 17th, 2010

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Questo protocollo descrive un metodo non-virale di consegna di costrutti genetici per una certa area del cervello dei roditori viventi. Il metodo consiste di preparazione plasmide, fabbricazione micropipetta, chirurgia neonatale dei cuccioli di ratto, microiniezione del costrutto, e

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