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JoVE Journal Neuroscience
Entrega de genes ao cérebro pós-natal do rato por Non-ventricular Injection sHRNA e Eletroporação
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Entrega de genes ao cérebro pós-natal do rato por Non-ventricular Injection sHRNA e Eletroporação

Article DOI: 10.3791/2244-v • 10:35 min • September 17th, 2010
September 17th, 2010 •
1, 1,2, 1,2, 1
1Neuroscience Center, University of Helsinki, 2Faculty of Biological and Enviromental Sciences, University of Helsinki

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Este protocolo descreve um método não-viral de entrega de construções genéticas para uma determinada área do cérebro de roedores vivos. O método consiste na preparação de plasmídios, fabricação micropipeta, a cirurgia de rato neonatal filhote, microinjeção da construção, e

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Neurociência Edição 43 transfecção cérebro in vivo eletroporação microinjeção rato cirurgia estereotáxica
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