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JoVE Journal Biology
Profilering van methyl-en andere S-Adenosyl- L-Homocysteine-bindende eiwitten door Capture Compound-massaspectrometrie (CCMS)
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Profilering van methyl-en andere S-Adenosyl- L-Homocysteine-bindende eiwitten door Capture Compound-massaspectrometrie (CCMS)

Article DOI: 10.3791/2264-v • 17:12 min • December 20th, 2010
December 20th, 2010 •
1, 2, 1, 1, 2, 1, 1
1Department of Biochemistry / Analytics, caprotec bioanalytics GmbH, 2Institute of Organic Chemistry, RWTH Aachen University

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Capture Verbindingen zijn trifunctionele kleine moleculen om de complexiteit van het proteoom te verminderen door functionele omkeerbare klein molecuul-eiwit interacties, gevolgd door foto-crosslinking en zuivering. Hier gebruiken we een Capture Compound met

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Biochemie Capture Compound foto-verknopen klein molecuul-eiwit interacties methyltransferase S-adenosyl-l-homocysteine SAH S-adenosyl-l-methionine SAM functionele proteomics LC-MS/MS
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