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Protocole pour des essais de l'ARNi dans les moustiques adultes (A. gambiae)
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Protocole pour des essais de l'ARNi dans les moustiques adultes (A. gambiae)

Article DOI: 10.3791/230
July 4th, 2007 •
1, 1
1Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University

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July 4th, 2007

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Inverse des approches génétiques se sont avérées extrêmement utiles pour déterminer quels gènes de résistance sous-tendent à des agents pathogènes vecteurs de moustiques. Ce protocole vidéo illustre une méthode utilisée par le laboratoire Dimopoulos à injecter dans les ARNdb Anopheles gambiae moustiques, qui abritent le parasite du paludisme. La technique de manipulation de l'installation d'injection et injection ARNdb dans le thorax est illustré.

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