Induction of Neuroinflammation in Mice via Injection of Alpha-Synuclein Fibrils

For intraperitoneal injections, briefly narcotize the animals in an anesthesia chamber with isoflurane. Again, pinch the animal's toe and confirm that it does not withdraw its hindlimb to ensure proper anesthesia. Fill a 27-gauge disposable hypodermic syringe with 50 microliters of sonicated alpha-synuclein fibrils or PBS.

Hold the animal in a dorsal position, with the head facing away from the investigator, and downward at approximately 45 degrees. Directly inject into the peritoneum of the mouse without penetrating the small intestine or cecum located behind the abdominal wall. Monitor animals until they have recovered from the anesthesia.