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Journal / Neuroscience / Nucleofection e cultura primaria di neuroni…
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Neuroscience

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JoVE Journal Neuroscience
Nucleofection e cultura primaria di neuroni embrionali di topo ippocampale e corticale
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Nucleofection e cultura primaria di neuroni embrionali di topo ippocampale e corticale

Article DOI: 10.3791/2373-v • 15:40 min • January 24th, 2011
January 24th, 2011 •
*1, *1, *1, 1
1Department of Anatomy, University of Wisconsin-Madison
* These authors contributed equally

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Questo protocollo delinea i passaggi necessari per analizzare, attraverso trasfezione elettroporazione e neuroni dell'ippocampo e del mouse cultura corticale. Colture a breve termine possono essere utilizzati per gli studi della crescita degli assoni e la guida, mentre a lungo termine le culture possono essere utilizzati per studi di sinaptogenesi e analisi spine dendritiche.

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Neuroscienze Numero 47 ippocampo corteccia trasfezione nucleofection elettroporazione coltura primaria
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