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Journal / Neuroscience / La cultura del motore principale e neuroni…
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Neuroscience

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JoVE Journal Neuroscience
La cultura del motore principale e neuroni sensoriali in Media definiti in elettrofilate Poli-L-lactide Ponteggi nanofibre
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La cultura del motore principale e neuroni sensoriali in Media definiti in elettrofilate Poli-L-lactide Ponteggi nanofibre

Article DOI: 10.3791/2389-v • 16:03 min • February 15th, 2011
February 15th, 2011 •
1, 2, 3, 3, 3, 3, 3, 1,3,4
1Department of Biomedical Engineering, University of Michigan, 2State Key Laboratory of Bioelectronics, Southeast University, 3Department of Neurology, University of Michigan, 4Geriatric Research, Education and Clinical Center, Veterans Affairs Ann Arbor Health System

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Allineati fibre elettrofilate diretta la crescita dei neuroni in vitro e sono una componente potenziale di ponteggi rigenerazione nervosa. Abbiamo descritto una procedura per la preparazione di substrati di fibre elettrofilate e il siero senza cultura di primaria ratto E15 sensoriali (DRG) e neuroni motori. Visualizzazione dei neuroni mediante immunocitochimica è anche incluso.

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Neuroscienze Numero 48 Electrospinning motoneuroni nanofibre impalcatura colture cellulari primarie DRG
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