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Journal / Neuroscience / A Cultura da Motor Primário e neurônios…
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Neuroscience

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JoVE Journal Neuroscience
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A Cultura da Motor Primário e neurônios sensoriais no Media Definidas em electrospun Poly-L-ácido lático nanofibras Andaimes

Article DOI: 10.3791/2389-v • 16:03 min • February 15th, 2011
February 15th, 2011 •
1, 2, 3, 3, 3, 3, 3, 1,3,4
1Department of Biomedical Engineering, University of Michigan, 2State Key Laboratory of Bioelectronics, Southeast University, 3Department of Neurology, University of Michigan, 4Geriatric Research, Education and Clinical Center, Veterans Affairs Ann Arbor Health System

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Fibras alinhadas electrospun direcionar o crescimento de neurônios in vitro e são um componente potencial de andaimes regeneração nervosa. Nós descrevemos um procedimento para elaborar substratos fibra electrospun e da cultura livre de soro-sensorial primário de ratos E15 (DRG) e neurônios motores. Visualização de neurônios por imunocitoquímica também está incluído.

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Neurônios neurociência Edição 48 Electrospinning motor nanofibras andaime cultura de células primárias DRG
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