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Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones
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Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones

Article DOI: 10.3791/2409-v • 09:15 min • March 17th, 2011
March 17th, 2011 •
1, 1
1Department of Neuroscience, University of Minnesota

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A method to visualize and quantify F-actin barbed ends in neuronal growth cones is described. After culturing neurons on glass coverslips, cells are permeabilized with a saponin-containing solution. Then, a short incubation with the saponin buffer containing rhodamine-actin incorporates fluorescent actin onto free actin barbed ends.

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F-actin Barbed Ends Rhodamine-actin Permeabilized Neuronal Growth Cones Axons Molecular Guidance Cues Growth Cone Cytoskeleton Actin Filament Meshwork Actin Polymerization Dynamic Remodeling Guidance Cues Actin Monomer Polymerization Nuclei Chick Retinal Dorsal Root Ganglion (DRG) Actin Depolymerizing Factor (ADF/cofilin)
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