Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Development of a Negative Selectable Marker for…
JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection
Development of a Negative Selectable Marker for Entamoeba histolytica
Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time
Journal (Immunology and Infection)
Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time
Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
Journal (Immunology and Infection)
Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
 
Click here for the English version

Development of a Negative Selectable Marker for Entamoeba histolytica

Article DOI: 10.3791/2410-v • 06:05 min • December 12th, 2010
December 12th, 2010 •
1, 1, 1, 1
1Division of Infectious Disease and International Health, University of Virginia Health System

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

We report development of a negative selection system in E. histolytica based upon transgenic expression of a chimeric protein (FCU1) and selection with the prodrug 5-fluorocytosine. The FCU1 protein is a fusion of yeast cytosine deaminase and uracil phosphoribosyltransferase. Expression of FCU1 resulted in increased E. histolytica sensitivity towards 5-fluorocytosine.

Tags

Negative Selectable Marker Entamoeba Histolytica Amebiasis Gene Expression Transfection Plasmids Reverse Genetics Homologous Recombination Episomal Plasmid DNA Background Of Exogenous DNA Genomic Integration Event Negative Selection System Yeast Cytosine Deaminase And Uracil Phosphoribosyl Transferase Chimera (FCU1) Prodrug 5-fluorocytosine (5-FC) 5-fluorouracil 5-fluorouridine-5'-monophosphate
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter