Preparation of Cyanobacterial Culture for Biomass Production

Begin with a flask containing a pure culture of cyanobacteria, a photosynthetic bacteria.

Transfer the culture into a conical flask with a magnetic stir bar.

Add a buffered, nutrient-rich growth medium to supply nutrients and maintain optimal pH for cyanobacterial growth.

Incubate the culture with stirring under continuous white-light illumination and a constant supply of carbon dioxide.

Stirring ensures proper aeration and uniform distribution of nutrients.

Cyanobacteria absorb light energy and use carbon dioxide for photosynthesis to produce energy-rich products, supporting growth and division, and increasing their biomass.

Monitor the growth regularly. Once the culture reaches the exponential growth phase, transfer it into a centrifuge bottle.

Centrifuge to separate the cyanobacterial biomass. Discard the supernatant, which contains the spent growth medium.

The resulting cyanobacterial biomass is stored at an ultralow temperature for further experiments.

Begin by transferring the cell culture of 0.8 OD at 750 nanometers into a one liter flask and dilute it in 500 milliliters of B-HEPES medium to achieve the OD of 0.2 at 750 nanometers. Grow the culture with constant stirring at 30 degree Celsius until the OD reaches 0.8 to 1, indicating a late exponential growth phase. Centrifuge the culture at a speed of 10,000 times g for 20 minutes at room temperature and discard the supernatant.