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JoVE Journal
Neuroscience

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切片的制备,组织器官型培养,并记录在视交叉上核时钟基因活性的荧光素酶
 
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切片的制备,组织器官型培养,并记录在视交叉上核时钟基因活性的荧光素酶

Article DOI: 10.3791/2439-v 10:07 min February 15th, 2011
February 15th, 2011

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Summary

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Click here for the English version.

准备含有成年小鼠的下丘脑视交叉上核(SCN),和快速的方式来培养器官培养条件的SCN组织切片,程序报告。此外,使用动态的荧光素酶报告技术的振荡时钟基因蛋白表达的测量是描述。

Tags

神经科学杂志,48期,视交叉上核,小鼠,器官组织培养,昼夜节律,时钟基因,第2期,荧光素酶
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