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Immunology and Infection

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JoVE Journal Immunology and Infection
协议RBD - SARS疫苗的重组蛋白的制备，动物疫苗接种和中检测
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协议RBD - SARS疫苗的重组蛋白的制备，动物疫苗接种和中检测

Article DOI: 10.3791/2444-v • 12:10 min • May 2nd, 2011
May 2nd, 2011 •
1, 1, 1, 1
1Lindsley F. Kimball Research Institute, New York Blood Center

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这一协议描述为抗击非典研究重组受体结合域（RBD）为基础的亚单位疫苗的一般程序。它包括RBD的蛋白在293T细胞中，免疫与RBD的小鼠，检测小鼠血清中和使用建立非典pseudovirus中和试验活动的转染和表达方法。

Tags

免疫学，第51期，SARS受体结合域，亚单位疫苗，免疫接种，中检测
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