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Direkt Leverans av MIF Morpholinos Into the Zebrafish Otocyst med injektion och elektroporation Påverkar Inner Ear utveckling
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Direkt Leverans av MIF Morpholinos Into the Zebrafish Otocyst med injektion och elektroporation Påverkar Inner Ear utveckling

Article DOI: 10.3791/2466-v • 10:41 min • January 7th, 2011
January 7th, 2011 •
1, 2, 2, 2,3, 2,4
1Department of Veterinary Science, University of Wisconsin, Madison, 2Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, 3Present address: Department of Pulmonary Medicine, University of Michigan, Ann Arbor, MI, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI

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En metod för att leverera morpholinos direkt i zebrafisk otocyst på 24hpf har utvecklats. Använda mikroinjektion av morpholinos in i lumen öron vesikler och elektroporation att genomföra penetration, kunde vi kringgå effekten av morpholinos på hjärnan och få effekter som är specifika för innerörat.

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Utvecklingsbiologi Zebrafish innerörat mikroinjektion elektroporation morpholino
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