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JoVE Journal
Biology

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使用内源性野生型BRCA1基因的细胞表达,确定同源重组的BRCA1基因突变的影响
 
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使用内源性野生型BRCA1基因的细胞表达,确定同源重组的BRCA1基因突变的影响

Article DOI: 10.3791/2468-v 08:53 min February 17th, 2011
February 17th, 2011

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Summary

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Click here for the English version.

我们提供了一个用于测试在一个组织文化同源重组修复DNA损伤的检测消耗使用RNA干扰细胞内源性BRCA1蛋白和替换它与BRCA1基因点突变,其中包含一个编码变化的BRCA1基因变体的方法。

Tags

细胞生物学,第48期,BRCA1基因,同源重组,乳腺癌,RNA干扰,DNA修复
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