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Neuroscience
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本地化的蛋白质三维神经干细胞培养:一个混合的可视化方法
 
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本地化的蛋白质三维神经干细胞培养:一个混合的可视化方法

Article DOI: 10.3791/2483-v 21:47 min December 19th, 2010
December 19th, 2010

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Summary

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Click here for the English version.

在这里,我们描述了如何产生,扩大,并在三维(3D)培养的immunolabel产后海马神经祖细胞(筹备)。接下来,使用混合可视化技术,我们将演示如何immunolabelled冰冻切片的数字图像,可用于重建和地图的整个3D神经球免疫阳性细胞的空间位置。

Tags

第46期,神经科学,神经干细胞,海马,cryosectioning,3D建模,神经球,玛雅,合成
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