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分离翻译含肽- tRNA的核糖体的功能和结构分析
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分离翻译含肽- tRNA的核糖体的功能和结构分析

Article DOI: 10.3791/2498-v • 11:20 min • February 25th, 2011
February 25th, 2011 •
1, 1, 2, 1
1Department of Biological Sciences, University of Alabama Huntsville, 2Department of Biology, Stanford University

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含新生肽- tRNA的核糖体的生化分析的一个主要障碍已被其他核糖体的存在，在同一样品的核糖体不参与翻译的特定基因序列分析。我们制定了一个简单的方法，净化，完全包含利益的新生肽酰- tRNA，核糖​​体。

Tags

肽酰- tRNA的核糖体拖延，核糖体隔离，分子生物学，第48期，在体外翻译，RNA化学修饰，嘌呤霉素，抗生素。
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