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Journal / Bioengineering / Alta MicroRNA profiling Throughput: Multiplex…
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Bioengineering

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JoVE Journal Bioengineering
Alta MicroRNA profiling Throughput
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Alta MicroRNA profiling Throughput: Multiplex Ottimizzato qRT-PCR a scala nanoliter sul Fluidigm dinamica IFCS ArrayTM

Article DOI: 10.3791/2552-v • 07:27 min • August 3rd, 2011
August 3rd, 2011 •
1,2,3, 1,2,4, 5, 1,2,6, 1,2,3,7
1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, 2Center for Reproductive Sciences, University of California San Francisco, 3Department of Urology, University of California San Francisco, 4Department of Cell and Tissue Biology, University of California San Francisco, 5Fluidigm Corporation, Fluidigm Corporation, 6Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 7UCSF - Helen Diller Family Comprehensive Cancer Center, University of California San Francisco

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Qui descrivere un ottimizzato trascrittasi inversa multiplex PCR quantitativa (QRT-PCR) protocollo in combinazione con una piattaforma microfluidica come un efficace costi e tempi high-throughput screening per microRNA (miRNA) livelli di espressione, soprattutto quando si lavora con quantità limitate di campione.

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Bioingegneria Numero 54 microRNA qRT multiplex-PCR profilatura ad alto rendimento Fluidigm
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