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Journal / Bioengineering / उच्च Throughput MicroRNA रूपरेखा: अनुकूलित…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
उच्च Throughput MicroRNA रूपरेखा
A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
Journal (Bioengineering)
A Rapid and Quantitative Fluorimetric Method for Protein-Targeting Small Molecule Drug Screening
Preparing Limosilactobacillus reuteri DSM20016 Electrocompetent Cells for Electroporation
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उच्च Throughput MicroRNA रूपरेखा: अनुकूलित मल्टीप्लेक्स nanoliter स्केल पर Fluidigm गतिशील ArrayTM IFCs पर QRT - पीसीआर

Article DOI: 10.3791/2552-v • 07:27 min • August 3rd, 2011
August 3rd, 2011 •
1,2,3, 1,2,4, 5, 1,2,6, 1,2,3,7
1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, 2Center for Reproductive Sciences, University of California San Francisco, 3Department of Urology, University of California San Francisco, 4Department of Cell and Tissue Biology, University of California San Francisco, 5Fluidigm Corporation, Fluidigm Corporation, 6Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 7UCSF - Helen Diller Family Comprehensive Cancer Center, University of California San Francisco

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यहाँ हम एक समय और लागत प्रभावी उच्च throughput (miRNA) microRNA अभिव्यक्ति के स्तर के लिए स्क्रीनिंग उपकरण के रूप में एक microfluidic मंच के साथ एक अनुकूलित मल्टीप्लेक्स रिवर्स ट्रांसक्रिपटेस मात्रात्मक पीसीआर (QRT पीसीआर) संयोजन में प्रोटोकॉल का वर्णन है, खासकर जब नमूना की सीमित मात्रा में के साथ काम कर रहा है.

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बायोइन्जिनियरिंग 54 अंक microRNA QRT-पीसीआर मल्टीप्लेक्स उच्च throughput प्रोफाइलिंग Fluidigm
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