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Journal / Bioengineering / Hög genomströmning MikroRNA Profilering:…
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Bioengineering

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JoVE Journal Bioengineering
Hög genomströmning MikroRNA Profilering
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Hög genomströmning MikroRNA Profilering: Optimerad Multiplex QRT-PCR vid metod i nanoliter Skala på Fluidigm Dynamic ArrayTM IFCS

Article DOI: 10.3791/2552-v • 07:27 min • August 3rd, 2011
August 3rd, 2011 •
1,2,3, 1,2,4, 5, 1,2,6, 1,2,3,7
1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, 2Center for Reproductive Sciences, University of California San Francisco, 3Department of Urology, University of California San Francisco, 4Department of Cell and Tissue Biology, University of California San Francisco, 5Fluidigm Corporation, Fluidigm Corporation, 6Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 7UCSF - Helen Diller Family Comprehensive Cancer Center, University of California San Francisco

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Här beskriver vi ett optimerat multiplex omvänt transkriptas kvantitativ PCR (QRT-PCR) protokollet i kombination med en mikroflödessystem plattform som ett kostnadseffektivt och tidsbesparande high-throughput screening verktyg för mikro-RNA (miRNA) uttryck nivå, särskilt när man arbetar med begränsade mängder av prov.

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Bioteknik mikroRNA multiplex QRT-PCR hög genomströmning profilering Fluidigm
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