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Erkennung von Post-translationale Modifikationen auf Native Intact Nukleosomen mittels ELISA
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Erkennung von Post-translationale Modifikationen auf Native Intact Nukleosomen mittels ELISA

Article DOI: 10.3791/2593-v • 07:14 min • April 26th, 2011
April 26th, 2011 •
1, 2, 3, 2, 3
1Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 2Department of Molecular and Cell Biology, University of Connecticut, 3Department of Pharmaceutical Sciences, University of Connecticut

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Nucleosome ELISA (NU-ELISA) ist eine sensible und quantitative Methode zur globalen Muster der post-translationale Modifikationen in Zubereitungen native, intakte Nukleosomen zu erkennen. Diese Änderungen umfassen Methylierungen, Acetylierungen und Phosphorylierungen an bestimmten Histon Aminosäurereste und damit NU-ELISA bietet eine globale Proteom-Test des Gesamtsystems Chromatin Modifikation Staaten von spezifischen Zelltypen.

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Cellular Biology Ausgabe 50 Chromatin Nucleosome Epigenetik ELISA Histone Umbau Methylierung Acetylierung
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