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JoVE Journal
Neuroscience

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神经调节和线粒体运输:在较长的时间里,海马神经元的实时成像
 
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神经调节和线粒体运输:在较长的时间里,海马神经元的实时成像

Article DOI: 10.3791/2599
June 17th, 2011

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Summary June 17th, 2011

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Click here for the English version.

我们描述了一个协议,允许在较长的时间,通过荧光显微镜观察生活中的神经元线粒体成像。过长时间的成像是通过一个mitochondrially有针对性的荧光蛋白,并利用一种廉价的舞台上方的孵化器,在我们的实验室设计和内置的慢病毒介导的表达。

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