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Journal / Neuroscience / Neuromodulering och mitokondriell Transport:…
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Neuroscience

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JoVE Journal Neuroscience
Neuromodulering och mitokondriell Transport
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Neuromodulering och mitokondriell Transport: Bildproduktion i hippocampus nervceller under långa löptider

Article DOI: 10.3791/2599-v • 04:51 min • June 17th, 2011
June 17th, 2011 •
*1, *1, *1
1Department of Experimental Neurobiology, The Neurosciences Institute
* These authors contributed equally

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Vi beskriver ett protokoll som gör att avbildning av mitokondrierna i levande nervceller via fluorescensmikroskopi långa löptider. Imaging under längre perioder sker genom lentivirus-medierade uttryck för en mitochondrially riktade fluorescerande proteinet och användningen av ett billigt steg-topp inkubator som designades och byggdes i vårt laboratorium.

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Neurovetenskap mitokondrier transport Neuromodulering hippocampus neuron serotonin dopamin fluorescens Time-lapse Bildproduktion Stage-top inkubator
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