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JoVE Journal
Biology

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研究膜蛋白的构象动力学原位网站的荧光标记
 
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研究膜蛋白的构象动力学原位网站的荧光标记

Article DOI: 10.3791/2627-v 11:55 min May 29th, 2011
May 29th, 2011

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Summary

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Click here for the English version.

我们将介绍一种方法,措施以及具体站点的使用对单个细胞的荧光的构象变化的分析膜蛋白的离子转运的动力学。这种技术是适应离子通道,转运和离子泵并可以被用来确定蛋白亚基之间的距离限制。

Tags

第51期,细胞生物学,膜蛋白,两个电极电压钳,生物物理学,站点特定的荧光团的标签,显微镜,构象动力学
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