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JoVE Journal Neuroscience
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In kaart brengen en de toepassing van Enhancer-trap Flippase Expression in larvale en volwassen Drosophila CNS

Article DOI: 10.3791/2649-v • 09:45 min • June 3rd, 2011
June 3rd, 2011 •
1, 1, 1, 1, 1,2, 1, 1, 1, 1
1Department of Zoology, University of Oklahoma - Norman, 2Department of Biology, Brandeis University

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Beschrijven we een Flippase-geïnduceerde intersectionele Gal80/Gal4 repressie (FINGR)-methode, waardoor weefsel-specifieke Forever om Gal80 expressiepatronen te bepalen. Waar Gal4 en FLP elkaar overlappen, is Gal4 uitdrukking ingeschakeld (Gal80 omgedraaid out) of uit (Gal80 gespiegeld in). De FINGR methode is veelzijdig voor klonale analyse en neurale circuit in kaart brengen.

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Neurowetenschappen UAS Gal4 Gal80 Flippase FRT klonale analyse Gedrag Drosophila
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