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Etiquetado linaje de células de pez cebra con láser Uncagable fluoresceína Dextran
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Etiquetado linaje de células de pez cebra con láser Uncagable fluoresceína Dextran

Article DOI: 10.3791/2672-v • 07:36 min • April 28th, 2011
April 28th, 2011 •
1, 2, 2, 1
1Department of Biological Sciences, Vanderbilt University, 2Department of Chemical and Systems Biology, Stanford University

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Este protocolo esboza una manera de etiquetar y rastrear el destino de los pequeños grupos de células de embriones de pez cebra empleando la radiación UV-uncaging de fluoresceína enjaulado, seguido de monte se inmunomarcaje para amplificar la señal de la fluoresceína uncaged.

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Biología del Desarrollo número 50 el pez cebra el etiquetado linaje enjaulados fluoresceína los transgenes
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