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Journal / Bioengineering / Biomolecular Detection employing the…
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Bioengineering

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JoVE Journal Bioengineering
Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
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Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

Article DOI: 10.3791/2694-v • 11:05 min • May 3rd, 2011
May 3rd, 2011 •
1, 2, 2, 1, 2, 2, 3, 4,5, 4,5, 6, 6, 1, 5, 1,2
1Department of Electrical and Computer Engineering, Boston University, 2Department of Biomedical Engineering, Boston University, 3Center for Advanced Genomics Technology, Boston University, 4Department of Medicine, Section of Infectious Diseases, Boston University School of Medicine, 5Department of Microbiology, Boston University School of Medicine, 6CNR (National Research Council), Istituto di Chimica del Riconoscimento Molecolare

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Quantitative, high-throughput, real-time, and label-free biomolecular detection (DNA, protein, etc.) on SiO2 surfaces can be achieved using a simple interferometric technique which relies on LED illumination, minimal optical components, and a camera. The Interferometric Reflectance Imaging Sensor (IRIS) is inexpensive, simple to use, and amenable to microarray formats.

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Biomolecular Detection Interferometric Reflectance Imaging Sensor (IRIS) Biomolecular Interactions Diagnostic Applications Proteomic Biomarkers Genomic Biomarkers Molecular Affinities Molecular Specificities Labels Immunoassays Enzyme-linked Immunosorbent Assay (ELISA) Polymerase Chain Reaction (PCR) Gel Electrophoresis Assays Mass Spectrometry (MS) Label-free Methods
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