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Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells

Article DOI: 10.3791/2696-v • 09:18 min • June 29th, 2011
June 29th, 2011 •
1, 1
1Department of Genetics, The Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem

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We describe photobleaching methods including Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Loss In Photobleaching (FLIP) to monitor chromatin protein dynamics in embryonic stem (ES) cells. Chromatin protein dynamics, which is considered to be one of the means to study chromatin plasticity, is enhanced in pluripotent cells.

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Photobleaching Assays FRAP FLIP Chromatin Protein Dynamics Living Embryonic Stem Cells Protein Dynamics Study GFP Fusion Fluorescent Signal Recovery Time Lapse Imaging Mobility Analysis FLIP Assay
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