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JoVE Journal
Biology

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漂白实验(FRAP和FLIP)来衡量生活胚胎干细胞的染色质的蛋白质动力学
 
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漂白实验(FRAP和FLIP)来衡量生活胚胎干细胞的染色质的蛋白质动力学

Article DOI: 10.3791/2696
June 29th, 2011

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Summary June 29th, 2011

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我们描述漂白方法,包括荧光漂白后恢复(FRAP)和荧光漂白(翻转)监测胚胎干细胞(ES细胞)的染色质的蛋白质动力学的损失。染色质蛋白的动态,这被认为是研究染色质可塑性的手段之一,是增强多能干细胞。

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