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Immunology and Infection

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JoVE Journal Immunology and Infection
Não-cirúrgico instilação endotraqueal de camundongos com Análise de Pulmões e Drenagem Linfática Nodes Lung por citometria de fluxo
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Não-cirúrgico instilação endotraqueal de camundongos com Análise de Pulmões e Drenagem Linfática Nodes Lung por citometria de fluxo

Article DOI: 10.3791/2702-v • 08:02 min • May 2nd, 2011
May 2nd, 2011 •
1, 2, 1, 3, 1,2,4, 1,4
1Department of Immunology, University of Colorado School of Medicine, 2Division of Cell Biology, Department of Pediatrics, National Jewish Health, 3Department of Microbiology, Immunology, and Pathology, Colorado State University, 4Department of Immunology, National Jewish Health

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Nós ilustramos não-cirúrgico de entrega de materiais de teste para os pulmões de ratos anestesiados através da traquéia. Este método permite a exposição de pulmão a patógenos virais e bacterianas, citocinas, anticorpos, miçangas, químicos ou corantes. Iremos descrever colheita e processamento dos pulmões e dos gânglios linfáticos de drenagem de pulmão (LDLNs) para citometria de fluxo.

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Imunologia Edição 51 mouse intratraqueal pulmões gânglios linfáticos de drenagem de pulmão citometria de fluxo
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